Evaluation of the antioxidant and cytotoxic potency of Euphorbia rigida and Arbutus andrachne methanol extracts in human hepatocellular carcinoma cell lines in vitro

Abstract Background Ethnobotanical studies on plants and their active compounds take a great interest in traditional medicine. After pharmacological toxicological studies, there will be possibility to used therapy. This study aimed examine the vitro antioxidant cytotoxic activity of methanol extracts Arbutus andrachne L. Euphorbia rigida M.Bieb. 10, 25, 50, 75, 100 150 µg mL −1 concentrations A. E. were tested for by using DPPH radical scavenging assays, total capacity (phosphomolybdate assay) metal ion chelating activity. In addition, effects this Hep3B HepG2 human hepatocellular carcinoma cell lines evaluated at 24, 48 72 h. The cytotoxicity test was carried MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. Results Methanol extract obtained from both showed increased depending increase concentration. When compared free activity, found more effective than . assay revealed that except h treatment cells with 400 500 µgmL concentrations, did not show significant either or any concentration time. On contrary, it significantly proliferation exhibited statistically after treatment. However, as cells. Conclusions According our findings, determined have neither nor cells, while had especially Further research is needed identify purify ingredients extracts.